Composite

Part:BBa_K2737009:Design

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)


Pafe-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

We design afeR box upstream -35 region, and different site may have different efficiency.


Source

We add afeR box in front of biobrick BBa_I20170.

References