Composite
Part:BBa_K2737006:Design
Designed by: Xinyu Teng Group: iGEM18_ECUST (2018-10-06)
Plac-cecropin AD-T7 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 229
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence of cecropin AD is lack of initiation codon and termination codon.
Source
Coding sequence of cecropin AD is found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY328473 Combine Plac+RBS+ceropin AD+ T7 terminator.