Regulatory
Part:BBa_K2737003:Design
Designed by: Xinyu Teng Group: iGEM18_ECUST (2018-10-06)
constitutive promoter with fur box between -35 and -10 region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Interval sequence between -35 to -10 region fits into fur box.
Source
Promoter of lacI from plasmid pET28a+ replaced the sequence from -35 to -10 region with fur box GAATGATAATCATTATC