Part:BBa_K2732001:Design

Yeast Plasmid pYTK-AFA18

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal XbaI site found at 15
  Illegal SpeI site found at 1987
  Illegal PstI site found at 1996
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal SpeI site found at 1987
  Illegal PstI site found at 1996
  Illegal NotI site found at 4457
  Illegal NotI site found at 6315
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal BglII site found at 855
  Illegal BamHI site found at 1578
  Illegal XhoI site found at 1593
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal XbaI site found at 15
  Illegal SpeI site found at 1987
  Illegal PstI site found at 1996
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal XbaI site found at 15
  Illegal SpeI site found at 1987
  Illegal PstI site found at 1996
  Illegal AgeI site found at 2909
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 4098
  

Design Notes

Each part was flanked by the BsaI restriction enzyme sites in order to perform a one-pot Golden Gate assembly.

Source

These parts were derived from both E. coli and S. cerevisiae.