Part:BBa_K273000:Design

Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1015
  Illegal AgeI site found at 1162
  Illegal AgeI site found at 1540
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 49
  Illegal SapI site found at 463
  Illegal SapI site found at 1126
  

Design Notes

Km and hill values can be found in this article:

[http://dx.doi.org/10.1016/S0167-4838(98)00077-6 Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme. Judith M. Candy and Ronald G. Duggleby. Biochimicat Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology Volume 1385, Issue 2, 16 June 1998, Pages 323-338]

Source

Source of the Pyruvate decarboxylase (pdc) is Z. Mobilis ATCC 29191 via Phusion PCR with the following primers:

pdc_forward CGCgaattcgcggccgcttctagATGAGTTATACTGTCGGTACC 44nt

pdc_reverse CGctgcagcggccgctactagtaTTATTAGAGGAGCTTGTTAACAGGC 48nt

The strain was a kind gift from [http://www.fz-juelich.de/ibt/physio Microbial Physiology Group FZ Jülich, Dr. Stephanie Bringer]

BioBricked by the Uppsala iGEM Team 2009

References

[http://www.ncbi.nlm.nih.gov/pubmed/3546263 Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli. Neale AD, Scopes RK, Wettenhall RE, Hoogenraad NJ. J Bacteriol. 1987 Mar;169(3):1024-8]

[http://dx.doi.org/10.1016/S0167-4838(98)00077-6 Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme. Judith M. Candy and Ronald G. Duggleby. Biochimicat Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology Volume 1385, Issue 2, 16 June 1998, Pages 323-338]