Protein_Domain
Part:BBa_K2730000:Design
Designed by: Xinyue Ma Group: iGEM18_Tongji_China (2018-10-08)
Type III secretion chaperone-ExoS54-Flag-MSC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 429
Design Notes
Following the flag, there is sequence of MSC(SacI-KpnI-XbalI-SalI-HindIII) for adding.
Source
The domains of chaperone, promotor region and ExoS54 comes from natural Pseudomonas aeruginosa.