RNA

Part:BBa_K2725014:Design

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)


J23102 - supD - B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 80


Design Notes

J23102 positioned the same number of bases upstream from start of supD as pLac is in BBa_K914000.


Source

supD part sequence from BBa_K914000. Promoter sequence from BBa_J23102, terminator from BBa_B1006.

References