Coding

Part:BBa_K2725013:Design

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)


P1003 Casette with 10 Amber substitutions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

10/16 of the serine codons in the P1003 coding sequence were substituted for amber codons - distributed as evenly as possible throughout the coding sequence.


Source

Sequence from BBa_K914018 edited - more amber substitutions (detailed in description).

References