Part:BBa_K2721010:Design
the gene of csgA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We obtained this part by PCR amplification and added spytag in the N-terminal and 6Xhistag in C-terminal in the PCR primers. After verifying it by agarose gel electrophoresis，we assembled it into pSB1C3 and expressed the csgA in DH5alpha.We used the cnago red to dye the pellet and proved the expression.(Result is as follow)
Source
We obtained this part by PCR amplification from the genome of Escherichia coli MG1655.They we added the spytag in the N-terminal and 6Xhistag in C-terminal in the PCR primers.
References
[1] Edwin van Bloois, Remko T. Winter, et al. Decorating microbes: surface display of proteins on Escherichia coli.Cell, 2011,29(2):79-86 [2] Michelle M. Barnhart, Matthew R. Chapman. Curli Biogenesis and Function. Annu. Rev. Microbiol. 2006. 60:131–47