Coding

Part:BBa_K2719011:Design

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)


Collagen coding device with blue chromoprotein

For this part we used two reporter cassettes, both with their own promoter and a coding sequence of RFP and Blue chromoprotein. In addition, there is an IPTG inducible cassette for collagen V-like and what we wanted to prove with this combination was to create a more selective reporter system.

In the other hand, this part specifically is optimized for E. coli Rosetta (DE3) because it was the only strain modify for recognizing non-canonical amino acids. (Figure 1)

"T--TecCEM--BBa_K2719011Diagram.png"

Figure 1. Diagram of BBa_K2719011, leptin expression device.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 848
    Illegal NheI site found at 871
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1887


Design Notes

Collagen coding device with blue chromoprotein


Source

Collagen coding device with blue chromoprotein

References