Coding

Part:BBa_K2719010:Design

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)


Leptin coding device with blue chromoprotein

Here the part is composed by tow promoters, one for Blue Chromoprotein cassette and the other one for leptin (T7-lac induced by IPTG). Also, it has the coding sequences for blue chromoprotein and leptin and double terminator for both cases. The purpose of Blue chromoprotein was to simplify the selection of colonies. (Figure 1)

"T--TecCEM--BBa_K2719010Diagram.png"

Figure 1. Diagram of BBa_K2719010, leptin expression device



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Leptin codiNg device with blue chromoprotein


Source

Leptin codiNg device with blue chromoprotein

References