Part:BBa_K2719008:Experience

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Applications of BBa_K2719008

To confirm the presence of Leptin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1).

Figure 1. Colonies transformed with leptin coding site.

To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the extraction of the entire plasmid (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 3)

Figure 2. 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 11) Leptin Coding Site (BBa_K2719008) V'; 12) Leptin Coding Site (BBa_K2719008)V

Figure 3. 0.85% agarose gel with GelRed: MW) 2-Lod DNA Ladder; 6) Leptin Coding Site (BBa_K2719008) restricted with NotI

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