Coding

Part:BBa_K2718021

Designed by: Dimitri Popoff Emeline Lemarie   Group: iGEM18_Aix-Marseille   (2018-08-27)


Endochitinase 6-His Beauveria bassiana

This biobrick corresponds to the part BBa_K2718020 with a C-terminal 6-his-tag.For more information see BBa_K2718020 and BBa_K2718022

Design Notes

This biobrick is in RFC 25 standards with :

the prefixe (with ATG in frame) : 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC

and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Weiguo Fang, Bo Leng, Yuehua Xiao, Kai Jin, Jincheng Ma, Yanhua Fan, Jing Feng, Xingyong Yang, Yongjun Zhang, Yan Pei (2005).Cloning of Beauveria bassiana Chitinase Gene Bbchit1 and Its Application To Improve Fungal Strain Virulence Appl. Environ. Microbiol. Jan 2005, 71 (1) 363-370; DOI: 10.1128/AEM.71.1.363-370.2005

Rajasekhar, Pinnamaneni & Kalidas, P & Sambasiva Rao, K.R.S.. (2010). Cloning and Expression of Bbchit1 gene of Beauveria bassiana~!2009-03-20~!2010-04-10~!2010-06-30~!. The Open Entomology Journal. 4. 30-35. 10.2174/1874407901004010030.

Pedrini N, Ortiz-Urquiza A, Huarte-Bonnet C, Zhang S and Keyhani NO (2013) Targeting of insect epicuticular lipids by the entomopathogenic fungus Beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction. Front. Microbio. 4:24. doi: 10.3389/fmicb.2013.00024

Ferrari et al., A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection.Biotechnology for Biofuels 2014, 7:37

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