Composite
λcI

Part:BBa_K2717018:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


λcI(λ repressor-RBS2000-GDH-v5 tag-his tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2449
    Illegal AgeI site found at 3341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3323


Design Notes

We adjusted the promoter of GFP.

Source

GFP and GDH is stored in our own lab.

References

[1]Schwartz S, Felber B K, Pavlakis G N. Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.[J]. Molecular & Cellular Biology, 1992, 12(1):207-19. [2]Das A, Yanofsky C. Restoration of a translational stop-start overlap reinstates translational coupling in a mutant trpB′-trpA gene pair of the Escherichia coli tryptophan operon[C]// IEEE International Congress on Big Data. IEEE, 1989:46-53. [3]Szabo G, Katarova Z, Greenspan R. Distinct protein forms are produced from alternatively spliced bicistronic GAD mRNAs during development[J]. Molecular & Cellular Biology, 1994, 14(11):7535-7545.