Composite
λcI

Part:BBa_K2717018

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


λcI(λ repressor-RBS2000-GDH-v5 tag-his tag)

This part is improved from BBa_K2717023. In this part, we first use the sequence in part with the promoter from BBa_K1139150 . We design this part to examine the expression of CI protein and to construct the co-expression between CI and GDH with TGATG linkage.

Function：We design this part to test the expression of CI protein with v5-tag and both expression-activation and growth advantage can be detected under the induction of IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2449
    Illegal AgeI site found at 3341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3323


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Categories
Parameters
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