Composite

Part:BBa_K2717011:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


RBS2000-GFP-TGATG-GDH-v5 tag-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2292
    Illegal AgeI site found at 3184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3166
    Illegal BsaI.rc site found at 678


Design Notes

NONE


Source

The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves. The GFP are derived from the Kit.

References