Composite

Part:BBa_K2717010:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


gdh-gfp-reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1157
    Illegal AgeI site found at 2049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal BsaI.rc site found at 1175


Design Notes

NONE


Source

The Promoter J23100, RBS 2000 and GFP are derived from the Kit. The glucose dehydrogenase is isolated from the genome of the laboratory strain and V5 tag and His tag are obtained from our own lab.

References