Composite

Part:BBa_K2717009

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


RBS2000-scar-GFP

This part is a composite one, in which Promoter lacI and RBS B0034 control a GFP. We design this part as one of the control groups to calculate the efficiency of stop-start codon “TGATG” (named control group 1). We design this part to test the efficiency of stop-start codon "TGATG". Green fluorescence can be detected after using this part (with IPTG induction).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 677


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Categories
Parameters
None