Plasmid_Backbone

Part:BBa_K270002:Design

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)


Leucine Landing Pad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5821
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5827
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5821
    Illegal BamHI site found at 4647
    Illegal XhoI site found at 42
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5821
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5821
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5836
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2355
    Illegal SapI site found at 262
    Illegal SapI site found at 4473
    Illegal SapI.rc site found at 1333
    Illegal SapI.rc site found at 3437


Design Notes

This was a biobrick compatable vector available in the lab to place our parts on.


Source

constructed by former Ninfa lab member, Don Eun Chang

References