Coding

Part:BBa_K2696008:Design

Designed by: Makayla Schacht   Group: iGEM18_Purdue   (2018-10-11)


Igk-flag-FRP-HRP(1-58)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 266


Design Notes

Source

References

Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563.