Coding

Part:BBa_K2690002

Designed by: David Doyle   Group: iGEM18_SUIS_Shanghai   (2018-10-09)


PvsB - Vibrio parahaemolyticus vibrioferrin synthesis gene B

This coding sequence is part of the biosynthetic gene cluster for the synthesis and transport of the siderophore vibrioferrin. It's specific function in this process to to code for an enzyme for amide bond formation between 2-ketoglutarate and L-alanine, or L-alanine and ethanolamine (Fujita et al., 2011).

Primers for Biobrick construction and PCR amplification.

Forward Primer

5’ – GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT CAC AAC GCT TTA TAC CTA ACC– 3’

Reverse Primer

5’ – GT TTC TTC CTG CAG CGG CCG CTA CTA GTA TTA TTA ACG TAC GCT TTT TTT GAT GGC – 3’

Notes: forward G-C content = 25/55 = 45%; reverse G-C content = 25/56 = 46%

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None