Regulatory

Part:BBa_K2688026:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-28)


LEE5_native_ΔTir


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 50


Design Notes

This plasmid was obtained following PCR mutagenesis of pSB1C3-LEE5_gfp_variant-1_inframe (BBa_K2688016) in order to delete the open reading frame corresponding to a fragment of tir gene, which yielded BBa_K2688017. The introduced point mutation was then PCR corrected, yielding this biobrick.



Source

LEE5 is a topic of research at the host lab, which provided us with the source plasmid pKK-LEE5-gfp.

References