Composite

Part:BBa_K2688023

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)

tetO_RBS_ler2

Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island.

This protein was used by Leh and collaborators to perform in vitro footprinting assays on LEE5 DNA region, and to analyse the activation of LEE5 promoter in the native enteropathogenic E. coli context by flow cytometry (Leh et al. mBIO, 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None