Composite

Part:BBa_K2688022

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)

tetO_RBS_ler1

Ler is a nucleoid associated protein encoded by LEE type III secretion system present in some pathogenic E. coli. H-NS and Ler belong to the same family of protein of proteins qualified as ‘silencers’ of foreign DNA expression, the « H-NS family ». However, it has been reported that Ler can counteract the H-NS silencing on the LEE island.


Our results show that Ler1 can be used to generate heterogeneity of LEE5 promoter (see BBa_K2688016 and BBa_K2688018) activity within E. coli population. Under some conditions (e.g. 50 ng/ml anhydrotetracyclin), a bimodal expression of LEE5 can be observed in E. coli harbouring pSB1C3-tetO-RBS-ler1 and an integrated copy of LEE5-gfp.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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