Composite

Part:BBa_K2688016:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 52

This plasmid was obtained following PCR mutagenesis of pSB1C3-LEE5*-gfp (BBa_K2688014) in order to delete 2 pb (‘AC’ which were present downstream tir’). Within pSB1C3-LEE5*Δ2-gfp, the open reading frames of tir’ and gfp are in the same frame. Our results indicate that gfp expression is improved when these two coding sequences are in the same frame, and that H-NS protein is still able to control the LEE5*Δ2 region. Of note, Ler proteins are able to activate LEE5 promoter.