Coding

Part:BBa_K2688000

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-08-01)


cpg2_cytosolic

Carboxypeptidase G2 (cpg2) coding sequence, without periplasmic localization signal.

This enzyme cleaves folates into pteroates and glutamate(s). It has broad specificity, acting on folic acid, reduced folate but also folate analogs like methotrexate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 844


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Categories
Parameters
None