Part:BBa_K2686006:Experience

The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, for the 60-mer and that sfGFP binds to the encapsulin.

User Reviews

UNIQ258312524ab05d12-partinfo-00000001-QINU UNIQ258312524ab05d12-partinfo-00000002-QINU