Composite

Part:BBa_K2683023

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


Valine tRNA Synthetase (ValRS) in pSB1C3

Valine tRNA synthetase is responsible for attaching the valine amino acid onto its tRNA to form an aminoacyl-tRNA [1]. The part has been codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These original designs were done by the 2017 Lethbridge iGEM team (http://2017.igem.org/Team:Lethbridge).

This part has been improved by inserting it into the pSB1C3 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2661


[edit]
Categories
Parameters
None