Composite

Part:BBa_K2683020:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The subparts and codon sequence have been optimized for use in E.coli .

Source

https://parts.igem.org/Part:BBa_K2443014

References

[1] Mermershtain, I., Finorov, I., Klipcan, L., Kessler, N., Rozenberg, H., and Safro, M. (2010) Idiosyncrasy and isentity in the prokaryotic phe-system: Crystal structure of E.coli phenylanyl-tRNA sythetase complexed with phenylalanine and AMP. Protein Science. 20, 160-167