![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2683014:Design
Designed by: Kristi Turton Group: iGEM18_Lethbridge (2018-10-08)
FitD Codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2601
Illegal BglII site found at 2793
Illegal BamHI site found at 2247
Illegal BamHI site found at 2349
Illegal BamHI site found at 2880
Illegal BamHI site found at 4362
Illegal BamHI site found at 6534 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 507
Illegal NgoMIV site found at 2040
Illegal NgoMIV site found at 2766
Illegal NgoMIV site found at 2946
Illegal NgoMIV site found at 3051
Illegal NgoMIV site found at 3099
Illegal NgoMIV site found at 4710
Illegal AgeI site found at 3786 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
As FitD has a relatively large coding sequence, special considerations for assembly was done. In order to get the entire sequence in the form of g-blocks, the sequences were designed into eight separate g-blocks that would be assembled by SLIC assembly.
Source
Boston, M. (2018). US Patent No. 0139968 A1. Washington, DC: U.S. Patent and Trademark Office.
References
[1] Molloy, D., Mayer, D., Giamberini, L and Gaylo, M. (2013) Mode of action of speudomonas fluorescens strain CL145A, a lethal control agent of Dreissend mussels (Bivalvaia: Dreissenidae). JOurnal of invertebrate pathology. 113, 115-121