DNA

Part:BBa_K2680554:Design

Designed by: Stephanie Do   Group: iGEM18_William_and_Mary   (2018-10-16)


UNS 10 Sequence, from Torella et al., 2013


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The UNS10 sequence is necessary for all William and Mary transcriptional units as it allows them to be put on a backbone. It is also in accordance with the biobrick standard.


Source

Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.

References