Coding
Part:BBa_K2680539:Design
Designed by: Stephanie Do Group: iGEM18_William_and_Mary (2018-10-15)
TlpA39
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 508
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 793
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1096
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. 1
Source
Organism: Salmonella typhimurium
TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1
Original sequence: Piraner, Dan I., et al.
References
1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.