Terminator

Part:BBa_K2680535

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-14)


Terminator.png

ECK120033736 terminator

  • ECK120033736 is the fifth strongest natural transcriptional terminator characterized in Chen et. al 2013 (see supplementary figure 1). "Terminator strength (TS) was determined from a simple assay that compares the expression of two fluorescent reporters, one placed before and one after the terminator."1 Marked as recombination-resistant in a spreadsheet from Christopher Voigt lab.2

References

1Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. (2013). Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 10(7), 659.

2Voigt Lab Terminator Plasmids Available from Addgene. (n.d.). Retrieved October 14, 2018, from https://www.addgene.org/synthetic-biology/voigt/terminators/

Secondary Structure

File:Mfold-K2680535-1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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