Composite

Part:BBa_K2680281:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G LsRK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1647


Design Notes

Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"In enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of the type II bacterial quorum sensing molecule (AI-2). This phosphorylation is required for AI-2 sequestration in the cytoplasm and subsequent derepression of AI-2-related genes necessary for quorum development."1

References

1Zhu, J., Hixon, M. S., Globisch, D., Kaufmann, G. F., & Janda, K. D. (2013, May 29). Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/23672516