Composite

Part:BBa_K2680279:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G LsrR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1008


Design Notes

Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.


Source

LsrR is upstream of the lsr operon and represses the transcription of the lsr operon and itself. 1


References

1. Xue, T., Zhao, L., Sun, H., Zhou, X., & Sun, B. (2009, July 28). LsrR-binding site recognition and regulatory characteristics in Escherichia coli AI-2 quorum sensing. Retrieved from https://www.nature.com/articles/cr200991