Composite

Part:BBa_K2680278:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G mf-Lon SsrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1930
    Illegal AgeI site found at 2014
    Illegal AgeI site found at 2220
    Illegal AgeI site found at 2245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI site found at 89
    Illegal BsaI.rc site found at 2502
    Illegal BsaI.rc site found at 2553


Design Notes

This specific sequence was codon-optimized for E. coli using the website www.jcat.de


Source

This sequence was adapted from Gur, Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". Synthesized by IDT.

References

Cameron DE, Collins JJ. (2014) Tunable protein degradation in bacteria. Nature Biotechnology, 32(12):1276–1281.

Gur E, Sauer RT. (2008) Evolution of the ssra degradation tag in mycoplasma: specificity switch to a different protease. Proceedings of the National Academy of Sciences,105(42):16113– 16118.