Composite

Part:BBa_K2680275:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G tsLambda-cI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 777


Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

Lambda-CI originates in bacteriophage lambda. Temperature-sensitive lambda-CI originates in Sussman & Jacob (1962).1

References

1Sussman, R. & Jacob, F. Sur un systeme de repression thermosensible chez le bacteriophage lambda d’Escherichia coli. C. R. Hebd. Seances Acad. Sci. 24, 1517–1519 (1962).