Composite

Part:BBa_K2680251:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-07-07)


3G mScarlet-I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 574
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 778


Design Notes

Forbidden restriction sites were checked for and removed if necessary.

Source

This part was created via PCR and Gibson Assembly from BBa_K2333428.

References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074