Primer

Part:BBa_K268021:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)

YGR236C -> GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2462
    Illegal PstI site found at 1226
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2462
    Illegal PstI site found at 1226
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2462
    Illegal BglII site found at 1016
    Illegal BamHI site found at 45
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2462
    Illegal PstI site found at 1226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2462
    Illegal PstI site found at 1226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

Primers included ATG site


Source

Promoter region from gene YGR236C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).


References