Primer

Part:BBa_K268020:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)

YGR213C -> GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2461
    Illegal PstI site found at 1225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2461
    Illegal PstI site found at 1225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2461
    Illegal BglII site found at 1015
    Illegal BamHI site found at 44
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2461
    Illegal PstI site found at 1225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2461
    Illegal PstI site found at 1225
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704


Design Notes

Primers included ATG site


Source

Promoter region from gene YGR213C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).


References