Primer

Part:BBa_K268019:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)


YOR186W -> GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2463
    Illegal PstI site found at 1227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2463
    Illegal PstI site found at 1227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2463
    Illegal BglII site found at 31
    Illegal BglII site found at 1017
    Illegal BamHI site found at 46
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2463
    Illegal PstI site found at 1227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2463
    Illegal PstI site found at 1227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

Source

Promoter region from gene YGR035C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).

References