Composite

Part:BBa_K2680115:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G pLuxR-pR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 214


Design Notes

BBa_R0063 was PCR'd to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

Derived from Bba_R0063, which was originally derived from Vibrio fischeri.

References

Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0063. Retrieved October 14, 2018, from https://parts.igem.org/Part:BBa_R0063