Composite

Part:BBa_K2680109:Design

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-08-02)


3G PcinAM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 304


Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

pCinAM is the promoter to CinAM, an optimized sensor from Meyer et al (2018).1

References

1Meyer, A. J., Segall-Shapiro, T. H., & Voigt, C. A. (2018). Marionette: E. coli containing 12 highly-optimized small molecule sensors. BioRxiv, 285866. doi:https://doi.org/10.1101/285866