Composite

Part:BBa_K2680057:Design

Designed by: Stephanie Do, Xiangyi Fang, Ethan Jones, Jessica Laury, Adam Oliver, Lillian Parr, Chengwu Shen, Tinh Son, Julia Urban, Yashna Verma, Hanmi Zou   Group: iGEM18_William_and_Mary   (2018-10-06)


Tight Repression 37C Temperature-Sensitive mf-lon ssra


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2903
    Illegal AgeI site found at 2987
    Illegal AgeI site found at 3193
    Illegal AgeI site found at 3218
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembled through 3G Hybrid DNA Assembly. For more information, see [http://2018.igem.org/Team:William_and_Mary/3G William and Mary's 3G Assembly page].