Plasmid_Backbone
Part:BBa_K268001:Design
Designed by: Nora Yucel Group: iGEM09_UChicago (2009-10-21)
pRS316
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041
Illegal NotI site found at 2009 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal BamHI site found at 2029
Illegal XhoI site found at 2080 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041
Illegal NgoMIV site found at 1668 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1079
Illegal BsaI.rc site found at 3453
Illegal SapI site found at 2370
Illegal SapI site found at 4474
Illegal SapI.rc site found at 926
Design Notes
Eliminated PstI, XbaI and SpeI restriction sites.
Source
From ATCC. Pubmed accession number U03442.