Plasmid_Backbone

Part:BBa_K268001:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)


pRS316


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2047
    Illegal PstI site found at 2041
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2047
    Illegal PstI site found at 2041
    Illegal NotI site found at 2009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2047
    Illegal BamHI site found at 2029
    Illegal XhoI site found at 2080
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2047
    Illegal PstI site found at 2041
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2047
    Illegal PstI site found at 2041
    Illegal NgoMIV site found at 1668
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1079
    Illegal BsaI.rc site found at 3453
    Illegal SapI site found at 2370
    Illegal SapI site found at 4474
    Illegal SapI.rc site found at 926


Design Notes

Eliminated PstI, XbaI and SpeI restriction sites.


Source

From ATCC. Pubmed accession number U03442.

References