Composite

Part:BBa_K2675056:Design

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-07)


sfGFP-LVAtag expression casette under the control of J23110 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23110 constitutive promoter + custom made RBS (BBa_K2675017) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong L3S2P56 Terminator (BBa_K2675030)

Source

DNA synthesis & Assembly

References