RBS

Part:BBa_K2675014

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-05)


Synthetic RBS designed for Tat-SAIRGA

This part is a synthetic RBS designed for Tat-SAIRGA (BBa_K2675004) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of Tat-SAIRGA (BBa_K2675004) under the control of the BBa_J23100 promoter in the composite part BBa_K2675044. 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	10020,84
dG_total (kcal/mol)	:	-4,65
dG_mRNA_rRNA (kcal/mol)	:	-3,12
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-1,49
Accuracy (warnings)	:	OK


REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None