Coding

Part:BBa_K2665021:Design

Designed by: Masaaki Shimazoe   Group: iGEM18_Kyoto   (2018-10-15)


SED1 secretion signal - SdrG N2 N3 domain - 6xHis tag - ybbr tag-Stalk Region - SED1 anchoring domai


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1236
    Illegal SapI site found at 1556
    Illegal SapI site found at 3358


Design Notes

a


Source

a

References