Composite

Part:BBa_K2664008:Design

Designed by: Areti-Efremia Mellou   Group: iGEM18_Macquarie_Australia   (2018-10-09)


Complete chlorophyll expression pathway


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1720
    Illegal NotI site found at 3751
    Illegal NotI site found at 9504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 304
    Illegal BglII site found at 960
    Illegal BglII site found at 1155
    Illegal BglII site found at 4456
    Illegal BglII site found at 4580
    Illegal BglII site found at 6580
    Illegal BamHI site found at 352
    Illegal BamHI site found at 10997
    Illegal BamHI site found at 16476
    Illegal BamHI site found at 17457
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3031
    Illegal NgoMIV site found at 3850
    Illegal NgoMIV site found at 4392
    Illegal NgoMIV site found at 5138
    Illegal NgoMIV site found at 6804
    Illegal NgoMIV site found at 12155
    Illegal AgeI site found at 5784
    Illegal AgeI site found at 7302
    Illegal AgeI site found at 7356
    Illegal AgeI site found at 7575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 11159
    Illegal BsaI.rc site found at 6906
    Illegal BsaI.rc site found at 7701
    Illegal BsaI.rc site found at 9832
    Illegal BsaI.rc site found at 9988
    Illegal BsaI.rc site found at 10609
    Illegal BsaI.rc site found at 14945
    Illegal SapI.rc site found at 7631


Design Notes

trc promoter added to parts. combination of previously used parts and newly synthesised ones


Source

All genes were obtained from the algae Chlamydomonas reinhardtii and were codon optimised for expression in Escherichia coli.

References