Coding

Part:BBa_K2663004:Design

Designed by: Elizabeth Johnson   Group: iGEM18_WashU_StLouis   (2018-10-17)


AvrSr35 Avirulence Factor Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2467
    Illegal BamHI site found at 2594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 2636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 566
    Illegal SapI site found at 1767


Design Notes

The sequence for AvrSr35 was codon optimized for Saccharomyces cerevisiae EBY100.


Source

The sequence of this part comes from Puccinia graminis f. sp. tritici and registry DNA.

References